Figure 4.

Guanine supplementation alters the yeast ISR for eIF2B(γK66R)-containing cells

(A) Growth of eIF2Bγ mutants on selective medium containing or without guanine or adenine in the absence or presence of 3AT. Supplementation with guanine or adenine suppress the growth defect of K66R only.

(B) (Left) Cartoons summarizing impact of changing eIF2B activity on Gcn4 and His4-lacZ. Green arrows show high activity red arrows show low activity. (Right) β-galactosidase activity of His4-lacZ for indicated mutants in GCN2 cells with 3AT and/or guanine supplements shown. n = 3 ± s.e.m. t test, one-tailed ∗P < 0.05, ∗∗P < 0.01.

See also Figure S5.