Figure 5.

eIF2B(γK66) mutants impact eIF2B responses to GTP

(A) Co-IP of eIF2 with flag-eIF2B ± 1 mM GTP from soluble protein cell extracts performed and quantification of relative eIF2 enrichment ±s.e.m. (n = 3). t test, one-tailed, ∗p < 0.05, ∗∗p < 0.01.

(B) Coomassie-blue-stained gel of Flag affinity purified eIF2B complexes (1 μg).

(C) Binding of GTP to 12 μmol purified eIF2B proteins (n = 2)

(D) Effect of GTP on eIF2B GEF activity. Activity of 0.5 μg each protein—GTP is normalized to 100% to show the impact of prebinding GTP (n = 3). t test, one-tailed, ∗p < 0.05, ∗∗p < 0.01.

See also Data S1.