Figure 3.

Electrostatic interactions account for phase separation and photoswitching abilities

(A) Photoswitchable droplet formation at pH 7.0 in contrast to dispersed states at pH 12.5 (20 mM buffer concentration) (scale bar: 10 μm).

(B) Phase diagrams illustrating phase-separated states of trans and cisAAP-ODN. Empty circles represent phase-separated states; crosses represent non-phase-separated states (dispersed states). Plot areas are shaded only as a visual guide.

(C) Images of the energy minimum structures of trans and cisAAP-ODN with 20-mer pLys obtained through molecular dynamics simulation. End-to-end distance with simulation time is plotted; red and blue lines are distances from individual runs of trans and cisAAP-ODN respectively, with a total of six runs each; black and gray lines represent averages of the respective runs; dashed lines bound production runs.

(D) Interactions of AAP with neighboring bases in AAP-ODN. AAP is indicated in pink and hydrophobic interactions are shown as dashed lines.

See also Figure S2D.