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. 2021 Nov 15;11:775418. doi: 10.3389/fonc.2021.775418

Figure 2.

Figure 2

Berberine decreased m6A modification of total RNAs in CSCs. (A) METLL3, METLL14, FTO, WTAP, YTHDF1, and ALKBH5 mRNAs were analyzed in colorectal cancer samples compared with normal samples. (B) Protein levels of METLL3, METLL14, FTO, WTAP, YTHDF1, and ALKBH5 in CSCs and parental cells were measured by performing western blot. *p < 0.05, vs. PCs. (C) Total m6A levels in CSCs with or without Berberine treatment by EpiQuik m6A RNA Methylation Quantification Kit. RT-qPCR testing the mRNA levels (D) and Western blot examine the protein levels (E) of m6A regulating proteins. *p < 0.05, **p < 0.01, vs. 0 μM treat.