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Berberine promoted chemosensitivity. (A) Sulforhodamine B assay was performed to measured the toxicity of FIRI to HCT116 and HT29 CSCs. *p < 0.05, vs. mock group; #p < 0.05, vs. Berberine-treated group. (B) Annexin V-FITC/PI double staining was performed followed by flow cytometry to measure the apoptotic rate. *p < 0.05, vs. mock group; #p < 0.05, vs. Berberine-treated group. (C) Western blot was performed to detect apoptosis hallmarkers, cleaved PARP, and cleaved caspase-3.
