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. 2021 Nov 15;11:775418. doi: 10.3389/fonc.2021.775418

Figure 9.

Figure 9

Berberine decreased β-catenin and its downstream target genes. (A) After 12 or 24 h treatment using 10, 20, and 40 μM of Berberine, β-catenin protein levels were measured by Western blot in both HCT116 and HT29 CSCs. *p < 0.05, vs. mock group at 12 h; #p < 0.05, vs. mock group at 24 h. (B) After 12 or 24 h treatment using 10, 20, and 40 μM of Berberine, β-catenin mRNA levels were measured by RT-qPCR. After 40 μM of Berberine treatment, Axin2 and Cyclin D1 protein levels were measured by Western blot and RT-qPCR (C&D). *p < 0.05, vs. mock group; #p < 0.05, vs. mock group. After 40 μM of Berberine treatment, Axin2 and Cyclin D1 protein levels were measured by Western blot and RT-qPCR (C, D). *p < 0.05, vs. mock group; #p < 0.05, vs. mock group.**P < 0.01, vs. mock group at 24 h.