FIGURE 3.

mGluR3 labeling by IHC in young and aged rat layer II/III PL PFC. (A) Immunolabeling for mGluR3 in young rat layer II/III PL mPFC revealing high density of mGluR3 expression in multiple cell-types. (B) High-magnification micrograph showing mGluR3 expression in young rat layer II/III PL mPFC in excitatory pyramidal cells, and smaller putative astrocyte-like cells. The neuropil is also characterized by punctate-like labeling for mGluR3. (C) Expression of mGluR3 in aged rat layer II/III PL mPFC with a marked decrement in the density of labeling across cell-types. (D,E) High-magnification micrograph showing mGluR3 expression in aged rat layer II/III PL mPFC with sparser labeling, and decreased labeling in dendritic arbors, as well as possible labeling of reactive astrocytes, magnified in insert (E). Scale bars: 50 μm.