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. 2021 Nov 2;40(23):e108788. doi: 10.15252/embj.2021108788

Figure 4. Nup50 is a chromatin binding nucleoporin.

Figure 4

3 µM recombinant EGFP, EGFP‐tagged MEL28/ELYS (aa 2290–2408), or EGFP‐tagged Nup50 was incubated with empty or DNA‐coated magnetic beads, which were chromatinized with Xenopus egg extracts (right panel) or unchromatinized DNA beads (left panel). After 3 h, the beads were re‐isolated, washed, co‐stained with DAPI, and analyzed by confocal microscopy. Scale bar: 10 µm.