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. 2021 Oct 20;40(23):e108299. doi: 10.15252/embj.2021108299

Figure 2. Genome‐wide biases in amino acids distributions and van der Waals radii.

Figure 2

  • A
    Two‐dimensional plots of downstream translation continuation of the 5 aa‐EPDP constructs and averaged van der Waals molecular radii of inserted sequences. Both in vivo (left) and in vitro (right) experiments are represented with Spearman's rho and P‐values calculated by Spearman's rank correlation tests.
  • B
    Schematic of the ribosomal tunnel occupancy by the bulkiness of the nascent polypeptide adjacent to the PTC.
  • C
    Effect of the substitution of bulky (K and Y) residues of YeaD_5aa‐EPDP to smaller one (G) on IRD. The mean values ± SE estimated from three independent technical replicates are shown.
  • D
    Effect of the substitution of small (G) residues of YbaB_5aa‐EPDP to bulkier one (Y) on IRD. The mean values ± SE estimated from three independent technical replicates are shown.
  • E
    Distributions of the DE/P‐rich sequence (more than 3 DE/P in a 10‐residue moving window) appearance at the N‐terminal regions (left) and in the middle (right) of entire E. coli ORFs.
  • F
    Averaged van der Waals molecular radii of every 5‐aa window at the N‐terminal regions (left) and in the middle (right) of entire E. coli ORFs.