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. 2021 Oct 4;40(23):e108271. doi: 10.15252/embj.2021108271

Figure EV5. siRNA‐mediated knockdown of proteins in U‐2‐OS reporter cells.

Figure EV5

  1. CDKL5, ATM and ZMYND8 were depleted in U‐2‐OS 263 IFII reporter cells using indicated siRNA. siCON—non‐targeting control.
  2. qRT–PCR measurements in U2OS I‐PpoI cells to validate the silencing efficiency of siCDKL5 and the subsequent rescue efficiency of ectopic expression of siRNA‐resistant forms of CDKL5 wild‐type (WT) and K42R kinase‐dead mutant (KD). P2 and P3 primer pairs were used to analyse the changes in mRNA levels. The mean ± SD from two qPCR replicates of two independent experiments is shown.

Source data are available online for this figure.