Figure EV2. USP21‐CaaX expression does not alter the kinetics of TNF‐induced apoptosis or necroptosis in MDFs.

WT USP21, USP21‐CaaX and USP21^C221R‐CaaX were inducibly expressed in WT MDFs by doxycycline. TS and Nec‐1s were used to control for apoptotic signalling, and TSI was used to trigger necroptotic signalling. Sytox Green‐positive cells were quantified in real time by live cell imaging. Data are plotted as mean ± SEM of two independent experiments.

Data information: Wild‐type (WT), untreated (UT). TS and TSI are used as apoptotic or necroptotic stimuli respectively.

Source data are available online for this figure.