Figure EV4. N‐FLAG MLKL behaves like WT MLKL but does not induce cell death following necroptotic stimulation.

1. WT MLKL and N‐FLAG MLKL were inducibly expressed in Mlkl ^−/− MDFs by doxycycline for 12 h, and cells were treated with TSI or TSQ. TS treatment controlled that the response to TS was normal. Cell death was measured by PI staining based on flow cytometry. Data are plotted as mean ± SEM of three independent experiments.
2. WT MLKL and N‐FLAG MLKL were inducibly expressed in Mlkl ^−/− MDFs by doxycycline for 6 h, and cells were untreated (UT) or treated with TSQ. Crude membrane (M) and cytosolic (C) cellular fractions were analysed by Western blot from BN‐PAGE or SDS–PAGE using antibodies as indicated. Representative of three independent experiments.

Data information: Wild‐type (WT). TS, TSI and TSQ are used as apoptotic or necroptotic stimuli.

Source data are available online for this figure.