Figure 6. Simultaneous arginine replacement of four ubiquitylation sites on the mMLKL 4HB domain does not prevent necroptosis‐induced ubiquitylation.

1. Cartoon of the N‐terminal region (residues 1–180) of mouse MLKL (PDB accession 4BTF [Murphy et al, 2013]) showing the four lysine residues identified from MS analysis as yellow sticks.
2. WT and 4KR mutant MLKL were inducibly expressed in Mlkl ^−/− MDFs by doxycycline for 6 h and cells were untreated (UT) or treated with TSI, followed by UBA pulldown. Representative of three independent experiments.

Data information: Wild‐type (WT), whole cell lysate (WCL). TSI is used as a necroptotic stimulus.

Source data are available online for this figure.