Figure 2.

Dilazep suppresses cell cycle and the c-MYC transcriptional program. (A) Using GSEA, we analyzed our dilazep signatures (derived from treatment with 50 µM dilazep for 48 h) and found that, across all three PC cell lines tested, the most suppressed REACTOME gene sets were related to cell-cycle, mitosis and DNA replication. (B) Using GSEA, we analyzed our dilazep signatures and found that, across all three PC cell lines tested, the most suppressed HALLMARK gene sets were related to cell-cycle progression (E2F Targets and G2/M Checkpoint), downstream targets of c-MYC signaling (Myc Targets v1 and Myc Targets v2), and DNA Repair, highlighting dilazep as a potentially beneficial compound for inhibition of PC growth, regardless of androgen-dependence. All P < 0.001. A full color version of this figure is available at https://doi.org/10.1530/ERC-21-0085.