Figure 4.

Bacterial proliferation is affected by Hfe. RPMI was spiked with 10% sera of naïve mice of the indicated genotypes. Spiked RPMI was inoculated with wild-type (WT) S. enterica Typhimurium (S. Tm.) and its isogenic derivatives mutated in one of three or all three iron uptake systems (entC, sitABCD, feo). Where applicable, deferasirox (DFX), ferrous sulfate (FeSO⁴) and recombinant murine Lcn2 (rmuLcn2) was added. Liquid cultures were assessed for extracellular bacterial proliferation (G to I) using the optical density at 600 nm (OD600). **P<0.01, ***P<0.001 for the comparison between mouse genotypes, ^#P<0.05, ^##P<0.01 and ^###P<0.001 for the comparison to solvent (Ctrl) or the S. Tm. WT strain as applicable. n=4-6 independent experiments.