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. 2020 Oct 13;106(12):3149–3161. doi: 10.3324/haematol.2019.241745

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Figure 5. — Cell-type specific effects of Hfe on protein expression in the spleen. Spleen homogenates were prepared to quantify the expression of iron and immune relevant proteins by enzyme-linked immunosorbent assay. Protein levels of Fpn1 (A), H-Ft (B), Tfr1 (C), Lcn2 (D), IFN-g (E) and Nos2 (F), normalized for total protein content, are depicted as means ± standard deviations. Statistically significant differences as calculated by unpaired, two-sided student t-test are indicated. n=10 for Hfe^+/+, n=12 for Hfe^-/-, n=20 for AlfpCre^– Hfe^fl/fl, n=14 for AlfpCre⁺ Hfe^fl/fl, n=15 for LysMCre^– Hfe^fl/fl, n=15 for LysMCre⁺ Hfe^fl/fl.