Figure 6.

PVRIG is constitutively trafficked to the naural killer cell surface. (A) Poliovirus receptor-related immunoglobulin domain-containing (PVRIG) and (B) CD69 expression on isolated natural killer (NK) cells incubated alone, with K562 cells (1:1 ratio), or with plate-bound anti-CD16 antibody at 37ºC for the indicated time points. Representative data (mean ± standard deviation [SD] of duplicates) of two experiments is shown. (C and D) PVRIG expression on isolated NK cells incubated either (C) alone or (D) with plate-bound anti-CD16 antibody at 37ºC for the indicated time points, in the presence or absence of monensin (mon) or brefeldin A (BFA). Representative data (mean ± SD of duplicates) of two experiments is shown. Significance determined by multiple t-tests with Holm-Sidak’s correction, *P< 0.05 compared with NK alone (A and B) or untreated (C and D).