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. 2021 Nov 30;11(11):e053321. doi: 10.1136/bmjopen-2021-053321

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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HPLC-DAD chromatogram of an OJ from a commercially available OJ concentrate naturally rich in hesperidin (A), a control beverage with an identical total sugar concentration (B) and a control beverage additionally supplemented with hesperidin (C). Flavonoids were extracted according to IFU²⁹ and analysed by HPLC-DAD using a C18 column (250×4.6 mm, particle size 5.0 µm, Kinetex, Phenomenex, Aschaffenburg, Germany) and an acetonitrile-based elution gradient. HPLC-DAD, high-pressure liquid chromatography – diode array detection; OJ, orange juice.