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. 2021 Nov 15;12:789454. doi: 10.3389/fimmu.2021.789454

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Copyright © 2021 Nabi, Lewin, Collantes, Chouljenko and Kousoulas

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of antigen-specific memory T cell expansion in LN at 5DPI. (A) Representative histogram (top panel) and percentage of BrdU incorporation in CD19, CD4, CD8 and γδT cells in mLN cells using flow cytometry (bottom panel). (B) Representative gating (Top panel) and percentage of BrdU-positive TCM in CD4, CD8 and γδT cell populations. (C) Percentage of BrdU-positive γδT cell in the eye following infection. *<P=0.05, **<P=0.005 using Mann-Whitney test, n=5/group. ns, Non-Significant.