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. 2021 Nov 15;8:739212. doi: 10.3389/fcvm.2021.739212

Figure 2.

Figure 2

(A) Immunoblots of PAR2 and GAPDH are demonstrated. Columns indicate relative expression levels of PAR2 in macrophages transfected with PAR2-siRNA or scrambled siRNA. (B) Immunoblots of PAR2, iNOS, and GAPDH are demonstrated. Columns indicate the relative expression levels of iNOS and PAR2 in macrophages exposed to FXa at 0, 0.5, 1.0, and 2.0 U/L. Macrophages treated with FXa at 2.0 U/L were also transfected with PAR2-siRNA and scrambled siRNA. (C) Immunofluorescence stains of PAR2, iNOS, DAPI, and their merged images are demonstrated. Columns indicate the MFI of PAR2 and iNOS in macrophages exposed to FXa at 0, 0.5, 1.0, and 2.0 U/L. Macrophages treated with FXa at 2.0 U/L were also transfected with PAR2-siRNA and scrambled siRNA. (D) Columns indicate the concentrations of IL1, IL6, and TNFα in the cell culturing medium of macrophages exposed to FXa at 0, 0.5, 1.0, and 2.0 U/L. Macrophages treated with FXa at 2.0 U/L were also transfected with PAR2-siRNA and scrambled siRNA (data were presented as mean ± SD, n = 6, Student's t-test and ANOVA, *P < 0.05).