Figure 4.

(A) Schematic diagram of the non-contact and contact co-culturing models of macrophages and VSMCs is demonstrated. VSMCs in the contact co-culturing model were co-cultured with FXa- exposed macrophage transfected with or without Dll4-siRNA. (B) Columns indicate the detected concentrations of inflammatory cytokines including IL1, IL6, and TNFα in the non-contact and contact co-culturing model systems. (C) Immunofluorescence stains of MYH11 and DAPI and their merged images of VSMCs. Columns indicate the MFI of MYH11 and collagen content of VSMCs from the non-contact and contact co-culturing models. (D) Immunoblots of NICD1, HES1, and GAPDH. Columns indicate the relative expression levels of NICD1 and HES1 in VSMCs from the non-contact and contact co-culturing models (data were presented as mean ± SD, n = 6, Student's t-test and ANOVA, *P < 0.05).