Figure 1.

RNA-sequencing (RNA-Seq) indicates intrinsic apoptosis-related pathways upregulated in chlorpyrifos (CPF)-exposed primary cortical neurons. Primary cortical neuron cultures were established from C57BL/6J (WT) embryos (E14.5-16.5). In order to detect the biological changes that were occurring in neurons prior to death, the cultures were treated with either vehicle (DMSO) or a sublethal 30 µM concentration of CPF (see Supplementary Figure 1) for 6 or 24 h. We conducted an unbiased transcriptomic screen using RNA-Seq and analyzed the data using CLC Genomics. A, Heat map shows top 100 differentially expressed genes (DEGs) with a minimum fold change of 2.5-fold with a p value cutoff of .04. B, Panther Gene List Analysis was used to generate ontologic data from all DEGs changed 1.5-fold or more with CPF treatment with a p value of <.04 at both timepoints.