Figure 3 .

Fmr1 KO mice exhibit altered developmental regulation in the expression of GABA_AR α1 and α3 subunits in the auditory cortex. (A–C) Western blot analysis of membrane-bound expression of GABA_AR α3, GABA_AR α1, and GABA_AR α1:α3, with all timepoints normalized to P56 WT to analyze the developmental expression of the GABA_AR α-subunits in the auditory cortex. (A) Developmental expression of GABA_AR α3 exhibits an overall interaction between age and genotype, with significantly decreased expression for KO compared with WT at P12. (C) Developmental GABA_AR α1:α3 expression exhibits an overall interaction between age and genotype. (D–G) GABA_AR α-subunits expressed relative to age-matched WT littermates. (D) Fmr1 KO exhibit a trending decrease in GABA_AR α3 expression at P9 (n = 11 WT, 11 KO). (E) GABA_AR α3 is significantly decreased at P12 (n = 10, 11). (F) No differences in expression observed at P15 (n = 10, 10). (G) P24 Fmr1 KO exhibit a trending increase in GABA_AR α1, with significantly increased GABA_AR α1:α3 expression (n = 12, 11). No differences were observed at P56 (n = 18, 15), data not shown. (H) Representative western blots of GABA_AR α3 and GABA_AR α1 with β-actin used as loading controls. (I) Schematic of approximate auditory cortex dissections as described in Materials and Methods. ^*P < 0.05, ^**P < 0.01.