Figure 4 .

Decreased colocalization of GABA_AR α3 and VGAT in auditory cortex of P12 Fmr1 KO mice. (A) Representative confocal micrographs of immunohistochemistry of extracellular targeting GABA_AR α3 and presynaptic VGAT in L2/3. (B–D) Quantification of results using Coloc2 plugin from FIJI (n = 8 WT, 8 KO). Total fluorescence intensity of (B) GABA_AR α3 and (C) VGAT immunoreactivity. (D) Mander’s coefficient to calculate the fraction of VGAT immunoreactivity that colocalizes with GABA_AR α3. Two-way ANOVA indicates a significant genotype effect in (B) and (D); however, there were no significant differences in any particular cortical layer as analyzed by Sidak’s multiple comparisons test. No differences were found in VGAT intensity. ^**P < 0.01.