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. 2021 Nov 15;12:767508. doi: 10.3389/fimmu.2021.767508

Figure 3.

Figure 3

Cockroach allergen-activated AhR protects airway inflammation and Muc5ac production. (A) Histological examination of mouse paraffin lung sections stained with hematoxylin and eosin (H&E, upper panel) and Alcian Blue periodic acid-Schiff (AB-PAS, lower panel). (B) Representative immunofluorescence images of Muc5ac (red) and Muc5B (green) in the lung tissues of cockroach allergen-induced mouse model with wild-type (WT) and AhR-/- mice (n = 6). (C, D), RT-PCR analysis of Muc5ac (C) and Muc5B (D) in the lung tissues of cockroach allergen-induced mouse model (n = 6). (E) RT-PCR analysis of Muc5ac and Muc5B in cockroach extract (CRE, 50 µg/ml)-treated HBECs with or without AhR knockdown (n=6). (F) RT-PCR analysis of Muc5ac and Muc5B in CRE-treated HBECs with or without CH223191 (10 µM, n = 6). Data represent as medians with interquartile (IQR). *P < 0.05, **P < 0.01.