Skip to main content
. 2021 Nov 15;12:767508. doi: 10.3389/fimmu.2021.767508

Figure 5.

Figure 5

Epithelial AhR prevents cockroach allergen-induced airway epithelial mitochondrial ROS generation. (A) Intracellular ROS production in HBECs were detected by flow cytometry analysis with CM-H2DCFDA (n = 4). (B) Representative immunofluorescence images of ROS expression with dihydroethidium (DHE) in the lung tissues of cockroach allergen-induced mouse model with wild-type (WT) and AhR-/- mice. (C) Quantitative analysis of florescent signals [(B), n = 6]. (D) Representative immunofluorescence images of mitochondrial ROS expression with MitoSOX in cockroach extract (CRE, 50 µg/ml)-treated HBECs with or without AhR knockdown. (E) Quantitative analysis of florescent signals [(D), n = 6]. (F) Representative immunofluorescence images of mitochondrial ROS expression with MitoSOX in cockroach extract (CRE, 50 µg/ml)-treated HBECs with or without CH223191 (10 µM). (G) Quantitative analysis of florescent signals [(F), n = 6]. Data represent as medians with interquartile (IQR). *P < 0.05, **P < 0.01.