Table 3.
Phage-display derived anti-hIgG antibodies used for format comparison.
| Antibody clone | V-VH | D-VH | J-VH | V-VL | J-VL | Yield [mg/L] | EC50 [nM] | ||
|---|---|---|---|---|---|---|---|---|---|
| scFv-mFc | mIgG | scFv-mFc | mIgG | ||||||
| SH1842-A12 | IGHV1-18*01 | IGHD3-3*01 | IGHJ4*02 | IGLV2-11*01 | IGLJ3*01 | 140.0 | 15.2 | 0.46 | 0.35 |
| SH1842-D3 | IGHV3-9*01 | IGHD2-2*02 | IGHJ4*02 | IGLV7-43*01 | IGLJ3*02 | 152.8 | 58.8 | 0.25 | 0.23 |
| SH1842-D7 | IGHV3-30*18 | IGHD3-16*01 | IGHJ4*02 | IGLV1-40*01 | IGLJ3*01 | 108.0 | 24.8 | 0.12 | 0.11 |
| SH1842-G4 | IGHV3-30*18 | IGHD3-16*01 | IGHJ4*02 | IGLV3-21*03 | IGLJ3*01 | 143.2 | 15.6 | 1.90 | n.s.b. |
| SH1844-E3 | IGHV3-7*01 | IGHD4-23*01 | IGHJ3*02 | IGKV1-NL1*01 | IGKJ1*01 | 148.0 | 22.8 | 0.73 | 0.16 |
| SH1844-E11 | IGHV3-23*04 | IGHD1-26*01 | IGHJ4*02 | IGKV3D-11*01 | IGLKJ3*01 | 123.2 | 40.8 | 0.20 | 4.39 |
| SH1844-G6 | IGHV3-33*01 | IGHD2-21*01 | IGHJ4*02 | IGKV3-20*01 | IGKJ5*01 | 84.0 | 72.0 | 0.82 | n.s.b. |
| SH1846-F9 | IGHV1-46*03 | IGHD6-13*01 | IGHJ4*02 | IGKV1D-39*01 | IGKJ4*01 | 129.6 | 35.6 | 2.31 | n.b. |
Antibodies from various gene families were produced as mIgG2a-Fc fusion or full mIgG2a in Expi293F suspension cells. Yield values are extrapolated from 50 mL scale. “n.s.b.” indicates non-sigmoidal weak binding. No binding is abbreviated as “n.b.”.
The asterisk is typically used for indicating the allele of an antibody gene.