Fig. 5. Acd Incorporation into proteins in Live HEK Cells. Cells were transfected with the indicated plasmid for the protein of interest with a TAG codon and a plasmid encoding a dominant negative release factor to suppress protein truncation, with (+RS) or without (−RS) a plasmid encoding Mb AcdRS 82 and tRNACUA, confocal microscopy images show brightfield (BF), the Acd fluorescence channel (405 nm excitation; 409–480 nm emission), and the GFP (488 nm excitation; 500–621 nm emission) or YFP (514 nm excitation; 519–621 nm emission) channels. Scale bar = 20 μm. Transfection and imaging conditions are described in the ESI† confocal microscopy and FLIM section.
