Figure 5.

Schematic of the ORFs and encoded protein domain structures of segment 8 mRNAs and vRNA (PR8 strain). Zigzag lines represent splice junctions. Boxes represent ORFs in each of the three reading frames (Fr) or the major polypeptide species and their functional domains (partially shaded bars). AUG codons are represented by vertical lines, colored according to the predicted strength of their Kozak translation initiation context (green, strong; amber, intermediate; red, weak). Black arrows indicate AUGs known to be used for translation initiation for the indicated proteins. Gray arrows indicate AUG codons proposed to fire on the basis of T-cell epitope data (DRiPs). (A) Represents the unspliced transcript. (B) Represents mRNA2 (NEP). (C) Represents mRNA3 (NS3). (D) Represents vRNA (the putative NSP protein).