Piezo1 inhibitor, GsMTx4 (Gx), treatment is associated with reduced mechanical stress–induced osteoclastogenesis but not with TNF-α–induced osteoclastogenesis. Changes of mRNA profile of Cox2 (A), RANKL (C), OPG (D), and protein production of PGE2 (B) in hPDLCs after mechanical stress (12 hours) or TNF-α induction with or without addition of Gx. Values shown as mean ± SEM. n = 3, * P = .05; ** P = .01; *** P = .001; **** P = .0001.