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. 2021 Sep 22;11(12):3293–3303. doi: 10.1002/2211-5463.13295

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© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This article has been contributed to by US Government employees and their work is in the public domain in the USA

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 6 — Localization of CA covalently bonded to Cys side chains of HbS using Orbitrap LC‐MS/MS. m/z plots of b‐ and y‐ions for (A) αCys¹⁰⁴‐containing peptide, (B) βCys⁹³‐containing peptide, and (C) βCys¹¹²‐containing peptide of HbS, each with a + 178 mass shift relative to the unmodified Cys‐containing peptide.