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Cytochrome c oxidase activity with WT and mutant cytochrome c as electron donors. CcO activity was determined in mitochondria isolated from MEF cells upon addition of exogenous WT, K8Q, K8A, K53Q, and K53A Cc. Experimental data were normalized with respect to that obtained with WT Cc. Each value represents the average of three individual experiments ± SD. See the Methods section for details.
