Table 2.

Midpoint melting temperature (T _m) values for oxidized WT and mutant cytochrome c as calculated within the 185–250 nm (UV) and 300–600 nm (Vis) ranges by CD and fluorescence spectroscopy.

	T m (°C)
	CD spectra		Fluorescence
	185–250 nm	300–600 nm	185–250 nm
WT	55.5 ± 0.8/87.1 ± 0.2	62.4 ± 1.1/87.4 ± 0.3	48.6 ± 2.4/58.2 ± 0.9/93.4 ± 1.6
K8A	52.5 ± 0.2/86.5 ± 0.2	28.5 ± 0.8/56.1 ± 0.6/84.1 ± 1.2	53.2 ± 0.4/88.4 ± 0.4
K8Q	53.2 ± 0.3/88.2 ± 0.4	29.1 ± 0.3/69.1 ± 3.7/87.8 ± 0.7	51.2 ± 0.3/90.1 ± 0.3
K53A	57.7 ± 4.6/82.1 ± 1.7	49.0 ± 0.8/83.5 ± 0.2	52.4 ± 0.6/88.8 ± 2.5
K53Q	55.7 ± 0.4/87.8 ± 0.3	53.6 ± 0.5/87.0 ± 0.6	57.6 ± 0.5/92.5 ± 0.3