Table 4.

Midpoint redox potential, thermodynamic and kinetic parameters as calculated from electron transfer of WT, K8Q, and K53Q cytochrome c immobilized on a chemically modified polycrystalline gold electrode. $E_{\frac{1}{2}}$, Midpoint redox potential value, $\mathrm{\Delta }{S}_{\mathit{rc}}^0$, Reduction entropy, $\mathrm{\Delta }{H}_{\mathit{rc}}^0$, Reduction enthalpy, k _s, Electron transfer rate constant, A, Pre‐exponential factors, $\mathrm{\Delta }{H}_{\mathit{ET}}^{\#}$, Activation enthalpy.

	$E_{\frac{1}{2}}$ a (mV vs. NHE)	$\mathrm{\Delta }{S}_{\mathit{rc}}^0$ (J K−1·mol−1)	$\mathrm{\Delta }{H}_{\mathit{rc}}^0$ (kJ·mol−1)	k s (s−1)	10‐5·A (s−1)	$\mathrm{\Delta }{H}_{\mathit{ET}}^{\#}$ (kJ·mol−1)
WT	184 ± 5	–142 ± 8	–60 ± 6	1300 ± 50	3.1 ± 0.9	13.6 ± 0.5
K8Q	178 ± 5	–130 ± 7	–56 ± 4	1100 ± 50	12.1 ± 1.8	17.2 ± 0.8
K53Q	172 ± 5	–108 ± 8	–49 ± 5	1600 ± 50	9.7 ± 1.3	15.8 ± 0.7

^a Measured at 25 °C.