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Apoptosis of (A) A549 cells and (B) PC-9 cells induced by SHK. A549 cells and PC-9 cells were treated by various concentrations of SHK for 24 h; cells were stained with annexin V–fluorescein isothiocyanate/propidium iodide and evaluated for apoptosis by flow cytometry. All measurements were carried out in triplicates, and data are given as mean ± SD (n = 3). ** p < 0.01.
