FIGURE 4.

Cell viability of A549 and PC-9 cells. p53-overexpression plasmid (p53) or its control vector, p53 specific siRNA or siRNA NC, mimic 628-3p, inhibitor 628-3p, or their corresponding negative control was transfected into A549 or PC-9 cells by Lipofectamine 3000 as indicated. Then (A) total proteins were extracted, and p53 protein levels were determined on a Simple Wes System; (B) CCK-8 assay and (C) EdU assay were performed after 24 h of transfection. All measurements were carried out in triplicates, and data are given as mean ± SD (n = 3). ^* p < 0.05, ^** p < 0.01.