FIGURE 7.

SHK inhibits the growth and induces apoptosis of A549 and PC-9 cells through the p53/miR-628-3p pathway. A549 and PC-9 cells were pretreated with p53 siRNA or inhibitor 628-3p and then cotreated with 2.0 μM of SHK for 24 h; cells were stained with (A) EdU or (B) annexin V–fluorescein isothiocyanate/propidium iodide and analyzed by flow cytometry. siRNA NC and inhibitor NC were performed as negative control, respectively. All measurements were carried out in triplicates, and data are given as mean ± SD (n = 3). ^* p ≤ 0.05, ^** p ≤ 0.01.