FIGURE 4.

Relative expression levels of (A) β-1, 6-glucan synthase (PP4), (B) endochitinase (PH-1), and (C) endochitinase (chi18-15) genes were measured by qRT-PCR during the interaction of T. longibrachiatum (TG1) and F. pseudograminearum (Fg) in a dual plate assay at 150 mM salt stress. Gene expression was evaluated at three different interaction time points at Days 3 (T1), 7 (T2), and 14 (T3) after the interaction. The 2^–ΔΔct method was used for the relative expression of each target gene using the control and α-tubulin as the reference gene. Small bars represent the standard errors of the means. Different lower case letters indicate significant differences at P < 0.05 in Duncan’s multiple range test using two-way ANOVA.