FIGURE 4.

Loss of Ttyh1 leads to enhanced neurogenesis in adult mice. (A,B) Immunolabeling of cell-type-specific markers and quantification of immunopositive cells in the adult SVZ. The BrdU retaining cells, Sox2⁺GFAP⁺ NSCs, EGFR⁺ TAPs, DCX⁺ neuroblasts and CD24 ependymal cells were compared (A) and quantified (B) in SVZ between control and Ttyh1 KO mice at 2-months old (2 M). (C,D) Immunolabeling of cell-type-specific markers and quantification of immunopositive cells in the adult SGZ. The BrdU retaining cells, Sox2⁺GFAP⁺ NSCs, and DCX⁺ neuroblasts were compared (C) and quantified (D) in SGZ between control and Ttyh1 KO mice at 2 M. (E) Open field and Morris water maze tests between control and Ttyh1 knockout mice. Scale bar = 100 μm in A, C. n ≥ 4 for all experiments. Data are expressed as mean ± SEM. Statistical significance was calculated using an unpaired, two-tailed Student’s t-test. (∗∗∗) p < 0.001, (∗∗) p < 0.01, (∗) p < 0.05.