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PQS signal accumulation in WT and ΔpqsA cultures. PAO1 WT and ΔpqsA overnight cultures were washed once in M9 buffer and resuspended and incubated in buffer for a period of 5 minutes. PQS was quantified in spent media and in buffer against a PQS standard using a bio-reporter (Pseudomonas putida expressing β-galactosidase under control pqsR promoter). n = 6 per treatment; error bars represent ±1 SD; all groups are statistically different from each other, P < 0.05.
