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. 2021 Nov 16;12:761450. doi: 10.3389/fimmu.2021.761450

Figure 8.

Figure 8

miR-146a deletion results in increased Smad2, Smad3 and Smad4 recruitment to promoter. (A) Human and mouse promoters share five SBEs, three of which are identical and conserved in mouse and human. (B) miR-146a–/– and miR-146a+/+ B cells were stimulated with LPS or CD154 plus IL-4, IL-5, TGF-β, anti-δ mAb and RA for 72 h. The recruitment of Smad2, Smad3 and Smad4 to the promoter was analyzed by specific ChIP assays. Data are mean ± SEM from three independent experiments. **p < 0.01, *p < 0.05, unpaired t-test. (C) Cartoon depicting the increased availability of Smad2, Smad3 and Smad4 in miR-146a–/– B cells and increased recruitment of these transcription factors to the IgH locus promoter.