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. 2021 Nov 16;9:767051. doi: 10.3389/fcell.2021.767051

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Copyright © 2021 Fan, Ma, Chen, Zhou, Jiang, Zhang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of methyltransferase WTAP on proliferation, cell cycle and activation markers of HSCs. (A) Expression levels of WTAP in low-fibrosis and high-fibrosis samples derived from the GEO database. (B) Small interfering RNA of WTAP was screened by RT-qPCR assay. (C) Optimal stimulation time of WTAP small interfering RNA was screened by RT-qPCR assay. (D) Optimal stimulation concentration of WTAP small interference RNA was screened by RT-qPCR assay. (E) Cell proliferation was detected by CCK8 assay. (F) The phase of the cell cycle was detected by flow cytometry. a, control group. b, model group. c, si-WTAP group. d, si-NC group. (G) Quantification of the cell cycle results. (H) mRNA expression level of α-SMA. (I) The mRNA expression level of collagen Ⅰ. (J) Protein expression levels of α-SMA. (K) Protein expression levels of collagen Ⅰ. (L) Semiquantitative analysis of α-SMA protein. (M) Semiquantitative analysis of collagen Ⅰ protein.