Fig. 5. (a) Time-dependent anti-proliferative activity of 1a on human lung (NCI-H460), breast (MDA-MB-231) and colorectal (HCT116) cancer cells for 24, 48 and 72 h. Flow cytometric analyses of the (b) cell cycle progression (24 h) and (c) cell death (72 h) of NCI-H460 cells treated with 1a or 1d. (d) Time-dependent uptake of different platinum(ii) complexes in NCI-H460 cells. (e) Energy- or endocytosis-dependence of the uptake and (f) subcellular distribution of platinum content of 1a and 1d in NCI-H460 cells. (g) Cellular platinum content in 1a- and 1d-treated NCI-H460 lung cancer and CCD-19Lu normal lung fibroblast cells.
