FIGURE 7.

The effect of MSTN on the PI3K-AKT and ribosomal pathway. (A–C) Knockdown of MSTN expression resulted in a significant increase in mRNA expression level and protein expression level of majority differentially expressed proteins involved in PI3K-AKT and ribosomal pathway in GM, as well as a significant increase in the expression of key genes AKT1, RPS6, p-AKT1and p-RPS6 in the pathway. (D–F) The mRNA expression level and protein expression level of these proteins were also generally increased in DM3. These blots were cropped and original images were shown in Supplementary Figure S7 and Figure 8.