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. 2021 Nov 16;12:752129. doi: 10.3389/fgene.2021.752129

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Copyright © 2021 Sheng, Guo, Zhang, Zhang, Miao, Tan, Hu, Li, Ding, Li and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The physical interaction between MSTN and COL1A1. (A) Knockdown of MSTN expression in BSMSCs resulted in no significant change in COL1A1 mRNA expression, (B–C) but protein expression significantly increased in GM and DM3. (D) Co-IP against the endogenous MSTN using anti-MSTN antibody was conducted with the lysate from BSMSCs and the eluent was immunoblotted against anti-COL1A1 antibody. A normal rabbit IgG was used as a negative control for immunoprecipitation. These blots were cropped and original images were shown in Supplementary Figure S9.