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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nat Chem Biol. 2021 May 25;17(6):693–702. doi: 10.1038/s41589-021-00801-x

Figure 3. Rate enhancement is substrate-dependent.

Figure 3.

(A) Fluorescent SDS-PAGE gel depicting the production of SUMOylated substrate as a function of time with FKBP-RanGAP, FKBP-E2, and FRB-polySH33:polyPRM5 condensates with DMSO (−) or rapamycin (+). This gel is representative of 3 independent experiments.

(B) Quantification of data in panel A, showing intensity of the SUMOylated substrate band as a function of time. DMSO, black circles; rapamycin, red squares. Data are plotted as the mean and SD from n=3 independent experiments.

(C) Fluorescent SDS-PAGE gel depicting the production of SUMOylated substrate as a function of time with FKBP-RanGAP or FKBP-RanGAP*, FKBP-E2, and FRB-polySH33:polyPRM5 condensates with DMSO (−) or rapamycin (+). This gel is representative of 2 independent experiments.

All figure panels have associated raw data.