Extended Data Figure 1. Sensitivity of enhanced condensate activity to K_M, substrate concentration, and partition coefficient.

(A) Modeled ratio of total reaction rate in a phase separated solution, with and without recruitment of enzyme and substrate to the scaffold (Total_S and Total_US, respectively, as a function of substrate concentration (plotted as [S]/K_M,US) and partition coefficient (PC). Modeled for K_M,US = 70 and K_M,S = 17, as measured for FRB-polySH3₃+polyPRM₅, with identical PC values for enzyme and substrate. Modeling assumes simple, hyperbolic Michaelis-Menten kinetics (see Methods). Color scale is a relative representation of the z-axis values and goes from low (blue) to high (red). Inset is a plot of Total_S:Total_US rate as a function of substrate concentration at a fixed partition coefficient of 50.

(B) Modeled ratio of Total_S to Total_US as a function of PC and the change in K_M upon recruitment of enzyme and substrate to the scaffold, K_M,US/K_M,S. Total substrate concentration, [S]_T, set to 0.1 * K_M,US.

(C) Same as (B), except [S]_T set to 10 * K_M,US.