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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nat Chem Biol. 2021 May 25;17(6):693–702. doi: 10.1038/s41589-021-00801-x

Extended Data Figure 2. Total scaffold rate can be less than total unscaffolded activity in certain regimes if enzyme partitioning is much less than substrate partitioning.

Extended Data Figure 2.

(A)-(C) Modeled ratio of total reaction rate in a phase separated solution, with and without recruitment of enzyme and substrate to the scaffold as a function of substrate concentration and substrate partition coefficient (PCS). Both reactions have KM = 70. Enzyme partitioning (PCE) is 1 (A), 10 (B), and 100 (C); enzyme concentration, [E] = 0.1[S]. Model based on 0.01 droplet volume fraction.