(A) Modeled ratio of droplet and bulk reaction rates as a function of substrate concentration and partition coefficient (PC). Both reactions are scaffolded and have KM = 17. Enzyme partitioning is identical to substrate partitioning, and [E] = 0.1[S].
(B) Modeled fractional activity contributed by the droplet phase as a function of substrate concentration and partition coefficient (PC). Conditions same as in (A), with a 0.01 droplet volume fraction.