Skip to main content
. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Cell Rep. 2021 Nov 23;37(8):110051. doi: 10.1016/j.celrep.2021.110051

Figure 3. MHC class II+ ILC3s limit Th2 cell responses and allergic airway inflammation.

Figure 3.

(A) Representative plots and quantitative graphs of the frequency and number of Th2 cells in lung parenchyma and MedLN from H2-Ab1fl/fl control mice and MHC class IIΔILC3 mice after exposure to HDM or PBS. Cells were gated on live CD45+ CD19− CD3/CD5+ CD4+ (data pooled from 4 independent assays).

(B) Quantitative graphs of the numbers of eosinophils in lung parenchyma and BAL from mice in (A). Eosinophils were identified as live CD45+ SSC-Ahi, Siglec-F+ (data pooled from 4 independent assays, n = 8).

(C) ELISA analysis of sera from mice in (A) for HDM-specific IgG1 and total IgE (pooled from 2 independent assays, n = 4).

(D) Representative periodic acid-Schiff staining sections from lung of mice in (A). The scale bars represent 100 μm.

(E) Analysis of airway resistance on mice from (A) (pooled from 2 independent assays, n = 8). Data are represented as mean ± SEM. Statistics are calculated by two-way ANOVA (A and B) and AUC followed by one-way ANOVA (C and E). See also Figure S3.